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MHT CET Biology question

BiologyBiotechnology: Principles and ProcessesMHT CET

Which of the following statements are correct regarding Eco RI restriction endonuclease enzyme? 1. Eco.RI restriction endonuclease enzyme is isolated from Escherichia coli RY13. 2. Its recognition sequence is 5′ − GAATTC − 3′ / 3′ − CTTAAG − 5′ 3. Its site of cleavage is 5′ − G ↓ A − A − T − T − C − 3′ / 3′ − C − T − T − A − A ↑ G − 5′ (between G and A on each strand) Choose the appropriate option.

Answer: (C)

Solution

Eco RI is obtained from Escherichia coli RY13, recognises 5'-GAATTC-3' and cuts between G and A on

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Question ID VK-080261