MHT CET Biology question

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of [MHTCET 2019]

(A)Non-recombinant bacteria containing β-galactosidase
(B)Insertional inactivation of α-galactosidase in non-recombinant bacteria
(C)Insertional inactivation of α-galactosidase in recombinant bacteriaCorrect
(D)Inactivation of glycosidase enzyme in recombinant bacteria

Answer: (C)

Solution

In blue-white screening the foreign DNA inserted into the lacZ gene inactivates the galactosidase enzyme in recombinant colonies, so they cannot turn the chromogenic

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Question ID VK-080300