MHT CET Chemistry question

A mixture of two amino acids having pI 9.60 and 5.40 can be separated

(A)by adjusting the pH of the solution at 9.60
(B)by adjusting the pH of the solution at 5.40Correct
(C)by adjusting the pH of the solution at 7.0
(D)by adjusting the pH of the solution at 14.0

Answer: (B)

Solution

An amino acid does not migrate in an electric field at its own pI; at pH 5.40 the acid with pI 5.40 stays as a neutral zwitterion while the acid with pI 9.60 (pH

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Question ID VK-083574