NEET 2010 Biology question

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme

(1)5′ – GATATG – 3′ , 3′ – CTACTA – 5′
(2)5′ – GAATTC – 3′ , 3′ – CTTAAG – 5′Correct
(3)5′ – CACGTA – 3′ , 5′ – CTCAGT – 3′
(4)5′ – CGTTCG – 3′ , 3′ – ATGGTA – 5′

Answer: (2)

Solution

Restriction endonucleases recognise palindromic nucleotide sequences, in which the sequence of base pairs reads the same in the 5' to 3' direction on both strands. The sequence 5'-GAATTC-3' with the complementary strand 3'-CTTAAG-5' is the recognition site of EcoRI; reading the lower strand 5' to 3' also

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Question ID VK-090608