NEET 2011 Biology question
Read the following four statements (A-D) about certain mistakes in two of them (A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactal bumin enriched. (B) Restriction enzymes are used in isolation of DNA from other macro-molecules. (C) Downstream processing is one of the steps of R-DNA technology. (D) Disarmed pathogen vectors are also used in transfer of R-DNA into the host. Which are the two statements having mistakes ?
Solution
(A) is wrong: Rosie (1997) was the first transgenic cow (not buffalo) that produced human alpha-lactalbumin-enriched milk. (B) is wrong: restriction enzymes cut DNA at specific recognition sequences; isolation of DNA from other macromolecules is done by treating cells with lysing enzymes and removing proteins/RNA with
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