NEET 2026 Biology question

Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction :

(1)Extension Annealing Denaturation
(2)Annealing Denaturation Extension
(3)Denaturation Extension Annealing
(4)Denaturation Annealing ExtensionCorrect

Answer: (4)

Solution

Each PCR cycle: (i) denaturation - double-stranded DNA is heated (about 94 C) to separate the strands; (ii) annealing - primers bind to complementary regions of the single strands (about 50-60 C); (iii) extension - Taq polymerase adds

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Question ID VK-104906