NEET Biology question

BiologyBiotechnology: Principles and ProcessesNEET

The steps involved in the Southern blot test are as follows: I. X-ray film II. Electrophoresis III. Digestion with restriction enzyme IV. Ethidium bromide V. Radioactive probe Choose the option having the correct sequential order of these events.

Answer: (A)

Solution

The DNA must first be digested with a restriction enzyme (III), then separated by electrophoresis (II), visualised/transferred and probed, with ethidium bromide staining (IV)

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Question ID VK-125056