NEET Biology question
Consider the following statements: I. In the microinjection method, foreign DNA is directly injected into the nucleus of an animal or plant cell by using micro needles or micro pipettes. II. The microinjection method is used in oocytes, eggs and embryos. III. Electroporation is the formation of temporary pores in the plasma membrane of the host cell by using lysozyme or calcium chloride. IV. In the chemical mediated gene transfer method, certain chemicals such as CO help foreign DNA to enter the host cell. Which of the statements given above are correct?
Solution
Statement III is incorrect because electroporation uses a pulse of high voltage (not lysozyme or calcium chloride) to create transient pores, and statement IV is incorrect because the chemicals used in chemical-mediated gene
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