NEET Biology question

In recombinant DNA technology, a plasmid vector is cleaved by:

(A)Modified DNA ligase
(B)A heated alkaline solution
(C)The same enzyme that cleaves the donor DNACorrect
(D)A different enzyme than that which cleaves the donor DNA

Answer: (C)

Solution

A modified ligase or a heated alkaline solution would not generate compatible sticky ends, and using a different enzyme on the vector than on the donor DNA would create incompatible

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Question ID VK-125228