The correct sequence of making a cell competent is:
(A)Treatment with divalent cations -> Incubation of cells with recombinant DNA on ice -> Heat shock (42°C) -> Placing on iceCorrect
(B)Incubation of cells with recombinant DNA on ice -> Treatment with divalent cations -> Heat shock (42°C) -> Placing on ice
(C)Treatment with divalent cations -> Heat shock (42°C) -> Incubation of cells with recombinant DNA on ice -> Placing on ice
(D)Heat shock (42°C) -> Treatment with divalent cations -> Incubation of cells with recombinant DNA on ice -> Placing on ice
Answer: (A)
Solution
The cell must first be made competent by treatment with divalent cations (such as calcium), then mixed and incubated with the recombinant DNA on ice to allow the DNA to associate with the cell surface, and only then
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