NEET Biology question

BiologyBiotechnology: Principles and ProcessesNEET

The different steps of recombinant DNA technology are given below randomly. 1. Isolation of DNA fragments or genes to be cloned. 2. Introducing the recombinant DNA into a suitable cell (usually E. coli), called the host (transformation). 3. Multiplication/expression of the introduced gene in the host. 4. Selection of the transformed host cells and identification of the clone containing the desired gene/DNA fragment. 5. Insertion of the isolated gene into a suitable plasmid vector. Which of the following represents the correct sequence of steps?

Answer: (C)

Solution

The gene of interest must first be isolated (i), then inserted into a suitable plasmid vector (v), then the recombinant construct is introduced into the host cell by transformation (ii), after which the introduced

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Question ID VK-125263