NEET Biology question
The given flow chart depicts the steps to transfer a desirable gene of interest into a plant: Isolation of the desirable gene using restriction endonucleases and gel electrophoresis -> A -> B -> Screening of cells for transformation -> C -> Regeneration of plants from the transformed cells to get transgenic plants. Identify the missing steps (A, B and C) with regard to the following statements and select the correct option. 1. Joining of the desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule. 2. Selection of transformed cells. 3. Transferring the recombinant DNA molecules to the target cells.
Solution
After the gene is isolated, it must first be joined to a cloning vector to create a recombinant DNA molecule (A), then this recombinant DNA is transferred into the target plant cells (B), which are then screened
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