NEET Biology question

Which of the given statements is correct in the context of observing DNA separated by agarose gel electrophoresis?

(A)DNA can be seen in visible light
(B)DNA can be seen without staining in visible light
(C)Ethidium bromide stained DNA can be seen in visible light
(D)Ethidium bromide stained DNA can be seen under exposure to UV lightCorrect

Answer: (D)

Solution

DNA itself does not absorb or emit visible light and cannot be directly seen in an agarose gel without staining, which rules out (a) and (b). DNA fragments are visualised by staining the gel with a fluorescent intercalating dye, most commonly ethidium bromide, which binds DNA and

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Question ID VK-125291