NEET Biology question

Which of the following is **not** required in the preparation of a recombinant DNA molecule?

(A)Restriction endonuclease
(B)DNA ligase
(C)DNA fragments
(D)*E. coli*Correct

Answer: (D)

Solution

Constructing a recombinant DNA molecule in vitro requires only: a restriction endonuclease to cut both the vector and the donor/gene-of-interest DNA at specific sites to generate compatible (sticky or blunt) ends; DNA ligase to seal/join the cut fragments together via phosphodiester bonds; and the DNA

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Question ID VK-125293