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NEET Biology question

BiologyBiotechnology: Principles and ProcessesNEET

The basic procedure involved in the synthesis of a recombinant DNA molecule is depicted below: mammalian DNA and a plasmid are each cut, the mammalian DNA with restriction endonuclease 1 and the plasmid with restriction endonuclease 2, and the resulting fragments are joined to form a recombinant molecule. The mistake in the procedure is

Answer: (C)

Solution

For two DNA molecules to be joined into a functional recombinant DNA molecule, both the vector (plasmid) and the donor (mammalian) DNA must be cut with the **same** restriction enzyme (or with enzymes that generate identical, complementary overhangs), so that the sticky/blunt ends produced on each are mutually

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Question ID VK-125304