NEET Biology question

BiologyBiotechnology: Principles and ProcessesNEET

Refer to the given table and select the option which correctly fills blanks X, Y and Z.

| Restriction enzyme | Source | Recognition sequence and sites of cleavage | |---|---|---| | EcoR I | X | 5'↓G-A-A-T-T-C-3' / 3'-C-T-T-A-A-G-5'↑ | | Y | *Bacillus amyloliquefaciens* H | 5'↓G-G-A-T-C-C-3' / 3'-C-C-T-A-G-G-5'↑ | | Hind II | *Haemophilus influenzae* Rd | Z |

Answer: (B)

Solution

By the standard restriction-enzyme naming convention, "EcoRI" decodes as genus *E*scherichia, species *co*li, strain *R*Y13, first (*I*) enzyme isolated from it — so X = *Escherichia coli* RY13, which rules out (a) and (d) (both wrongly say "R245"). The enzyme recognising GGATCC/CCTAGG is BamHI, sourced from *Bacillus

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Question ID VK-125306