NEET Biology question
Study the following statements regarding recombinant DNA technology and select the **incorrect** ones.
1. Taq polymerase extends the DNA primers using dNTPs in the presence of Mg2+. 2. Antibiotic resistance genes are considered as desirable (target) genes in recombinant DNA technology. 3. DNA fragments are separated according to their charge only, in agarose gel electrophoresis. 4. Transformation is a procedure through which a piece of DNA is integrated into the genome of a host bacterium. 5. To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. 6. Downstream processing is one of the steps of the polymerase chain reaction.
Solution
Statement 2 is incorrect because antibiotic resistance genes (e.g., ampR, tetR) function as selectable markers used to identify and select transformed cells — in modern usage they are not the "desirable" genes being cloned; the desirable/target gene is the separate gene of interest (e.g., the human insulin gene).
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