NEET Biology question

Refer to the given dichotomous key and select the **incorrect** option regarding P, Q and R.

1. (i) It is a DNA-mediated (chemical) gene transfer → P (ii) It is a direct or vector-less gene transfer → go to 2 2. (i) Cells to be transformed are bombarded with high-velocity micro-projectiles coated with DNA → Q (ii) Foreign DNA is directly injected into the nucleus of the cell to be transformed → R

• (A)In P, cells are first made competent by treating with divalent cations such as calcium
• (B)In Q, membranes of cells are treated with calcium chloride, which creates poresCorrect
• (C)Alec Jeffreys cured mice that had inherited a neuromuscular disease using technique R
• (D)Q is the biolistic or gene-gun method, whereas R is microinjection