NEET Biology question

Which of the following statements is correct regarding the EcoRI restriction endonuclease enzyme? **[NEET 2018]**

(A)It is isolated from *Escherichia coli* RY13Correct
(B)Its recognition sequence is 5'-AAGCTT-3' / 3'-TTCGAA-5'
(C)It produces complementary blunt ends
(D)None of these

Answer: (A)

Solution

EcoRI is isolated from *Escherichia coli*, strain RY13 (hence its name: *E*scherichia *co*li *R*Y13, enzyme *I*), which is a well-established, directly verifiable fact — so (a) is a correct statement. Option (b) gives the recognition sequence of HindIII (5'-AAGCTT-3'), not EcoRI, whose actual recognition sequence is

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Question ID VK-125315