NEET Biology question

What is the criterion for DNA fragments' movement on an agarose gel during gel electrophoresis? **[NEET 2017]**

(A)The smaller the fragment size, the farther it movesCorrect
(B)Positively charged fragments move to the farther end
(C)Negatively charged fragments do not move
(D)The larger the fragment size, the farther it moves

Answer: (A)

Solution

DNA fragments carry a uniform negative charge-to-mass ratio from their phosphate backbone, so under an applied electric field in agarose gel electrophoresis, all fragments migrate toward the positive electrode, and the *rate* at which they move through the gel's pores depends on fragment size: smaller fragments thread

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Question ID VK-125316