NEET Biology question

DNA precipitation out of a mixture of biomolecules can be achieved by treatment with **[NEET 2009]**

(A)Chilled chloroform
(B)Isopropanol
(C)Chilled ethanolCorrect
(D)Methanol at room temperature

Answer: (C)

Solution

In standard nucleic-acid isolation protocols, purified DNA is precipitated out of an aqueous solution by adding chilled ethanol, which reduces the solubility of DNA (by disrupting its hydration shell) and causes it to come out of solution as visible, pellet-able strands/fibres. Chilled chloroform (a) is used

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Question ID VK-125324