NEET Biology question

How are transformants selected from non-transformants? **[NEET 2008]**

(A)Presence of more than one recognition site in the vector DNA
(B)Presence of alien DNA in the vector DNA results in insertional inactivation of a selectable marker
(C)The antibiotic resistance gene gets inactivated due to insertion of alien DNA
(D)Both (b) and (c)Correct

Answer: (D)

Solution

When foreign ("alien") DNA is inserted into a selectable marker gene (classically an antibiotic resistance gene) located within the vector, it physically disrupts that gene's coding sequence — this is "insertional inactivation of a selectable marker" (b), and since the marker in question is typically an antibiotic

See the full step-by-step solution

Create a free account to read the complete working, and practise questions like this in a timed test.

Create a free account

Question ID VK-125325