NEET Biology question

Which of the following techniques is most widely employed to check the progress of restriction enzyme digestion? **[NEET 2006]**

(A)Agarose gel electrophoresisCorrect
(B)Centrifugation
(C)Polyacrylamide gel electrophoresis
(D)PCR

Answer: (A)

Solution

Running an aliquot of a restriction digest on an agarose gel and staining it (e.g., with ethidium bromide) lets one directly visualise whether the DNA has been cut into fragments of the expected sizes, making agarose gel electrophoresis the simplest, most routine and most widely used method to monitor digestion

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Question ID VK-125327