NEET Biology question

BiologyBiotechnology: Principles and ProcessesNEET

**Statement 1:** An *E. coli* cell carrying pBR322 with a DNA insert at the BamHI site cannot grow in a medium containing tetracycline. **Statement 2:** The recognition site for BamHI is present in the tetR region of pBR322.

Answer: (A)

Solution

Both statements are true, and Statement 2 directly explains Statement 1: the BamHI recognition site in pBR322 lies within the tetR (tetracycline resistance) gene, so inserting foreign DNA at this site

See the full step-by-step solution

Create a free account to read the complete working, and practise questions like this in a timed test.

Create a free account

Question ID VK-125343