NEET Biology question

Which of the following shows correct chronological order of the events occurring during callus culture?

(A)Explant → Callus → Cell division → Addition of cytokinin → Cells acquire meristematic property
(B)Explant → Cell division → Callus → Addition of cytokinin → Cells acquire meristematic propertyCorrect
(C)Callus → Explant → Cell division → Addition of cytokinin → Cells acquire meristematic property
(D)Cytokinin addition → Explant → Callus → Cell division → Cells acquire meristematic property

Answer: (B)

Solution

During callus culture an explant is first placed on the medium, its cells then divide repeatedly to produce the unorganised mass called callus, and only subsequently is cytokinin added (or its ratio to auxin shifted) to make the callus

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Question ID VK-125375