NEET Biology question
Arrange the steps involved in the production of humulin in the correct sequence and select the correct option.
1. Synthesis of gene (DNA) for human insulin artificially 2. Culturing recombinant E.coli in bioreactors 3. Purification of humulin 4. Insertion of human insulin gene into plasmid 5. Introduction of recombinant plasmid into E.coli 6. Extraction of recombinant gene product from E.coli
Answer: (C)
Solution
The correct sequence is to first synthesize the insulin gene (1), insert it into a plasmid (4), introduce the recombinant plasmid into E. coli (5), culture the
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