JEE Main Chemistry question
A mixture of 3 amino acids — glutamic acid, serine and lysine — is separated out one by one in the following 3 steps: (i) adding acid until the pH becomes 3.22, (ii) adding base until the pH becomes 5.68, (iii) adding base until the pH becomes 9.74. What is the isoelectric point of serine?
Answer: 5.68
Solution
Each amino acid precipitates out (is fully separated) at its own isoelectric point, where it has zero net charge and minimum solubility; glutamic acid (acidic) is removed at the lowest pH step (3.22 taken as its pI, here rounded in related
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