MHT CET Biology question

Steps in DNA fingerprinting are Isolation of DNA Digestion of DNA by A Separation of DNA by B Transferring of DNA to C DNA hybridisation using D Detecting of hybridized DNA by E Complete the accompanying flowchart. A, B, C, D and E in the flowchart are

(A)A - Restriction endonuclease; B - Electrophoresis; C - Nitrocellulose or nylon; D - Labelled VNTR probe; E - AutoradiographyCorrect
(B)A - Electrophoresis; B - Restriction endonuclease; C - Nitrocellulose or nylon; D - Labelled VNTR probe; E - Autoradiography
(C)A - Restriction endonuclease; B - Electrophoresis; C - Labelled VNTR probe; D - Nitrocellulose or nylon; E - Autoradiography
(D)A - Restriction endonuclease; B - Electrophoresis; C - Nitrocellulose or nylon; D - Autoradiography; E - Labelled VNTR probe

Answer: (A)

Solution

DNA is cut by restriction endonuclease (A), fragments are separated by electrophoresis (B), transferred to nitrocellulose or nylon (C),

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Question ID VK-065681