NEET 2003 Biology question

During translation initiation in prokaryotes, a GTP molecule is needed in : -

(1)Formation of formyl-met-tRNA
(2)Binding of 30S subunit of ribosome with mRNA
(3)Association of 30 S-mRNA with formyl-met-tRNACorrect
(4)Association of 50 S subunit of ribosome with initiation complex

Answer: (3)

Solution

Prokaryotic initiation: the 30S subunit binds mRNA (with help of IF-1 and IF-3); initiation factor IF-2 complexed with GTP then brings the initiator formyl-methionyl-tRNA to the P site of the 30S-mRNA complex, making the 30S initiation complex - so GTP is needed in the association

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Question ID VK-089268