NEET 2014 Biology question

An analysis of chromosomal DNA using the southern hybridisation technique does not use

(1)Electrophoresis
(2)Blotting
(3)Autoradiography
(4)PCRCorrect

Answer: (4)

Solution

Southern hybridisation involves restriction digestion of DNA, separation of fragments by gel electrophoresis, transfer (blotting) to a nitrocellulose/nylon membrane, hybridisation

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Question ID VK-091600