NEET 2026 Biology question
Arrange the following steps of DNA fingerprinting in a correct sequence. A. Isolation of DNA and its digestion by restriction endonucleases. B. Hybridisation using a labelled VNTR probe. C. Transferring of separated DNA fragments to synthetic membranes. D. Detection of hybridised DNA fragments by autoradiography. E. Separation of DNA fragments by electrophoresis. Choose the correct answer from the options given below :
Solution
DNA fingerprinting steps: isolate DNA and digest with restriction endonucleases (A); separate fragments by gel electrophoresis (E); transfer (Southern blotting) the fragments onto a nylon or nitrocellulose membrane (C); hybridise
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